pathhunter detection kit Search Results


pathhunter chemiluminescence detection reagents  (DiscoverX corporation)
90
DiscoverX corporation pathhunter chemiluminescence detection reagents
Pathhunter Chemiluminescence Detection Reagents, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pathhunter chemiluminescence detection reagents/product/DiscoverX corporation
Average 90 stars, based on 1 article reviews
pathhunter chemiluminescence detection reagents - by Bioz Stars, 2026-04
90/100 stars
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pathhunter detection kit  (DiscoverX corporation)
90
DiscoverX corporation pathhunter detection kit
(A) A panel of orphan GPCRs was screened in the presence of 10-nM MaR1 or vehicle (0.1% ethanol) using the β-arrestin <t>PathHunter</t> GPCR system. The % activity = 100% × (mean RLU of test sample – mean RLU of vehicle control)/(mean RLU of vehicle control). (B–E) Ligand (MaR1)-receptor interaction was monitored using the CHO-β-arrestin system overexpressing LGR6 or GPR148. Results are mean ± SEM from 3 independent experiments. (B) LGR6 or GPR148 cells with MaR1. #P < 0.05, ##P < 0.01. MaR1 versus vehicle with LGR6 cells. *P < 0.05, ***P < 0.001. LGR6 versus GPR148. (C) LGR6 cells with MaR1 or MaR1 ME. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. (D) LGR6 cells with MaR1, MCTR1, MCTR2, or MCTR3. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. (E) LGR6 cells with MaR1, Rspo-2, or Rspo-2+MaR1. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. For D and E, the 6 groups (MaR1, MCTR1, MCTR2, MCTR3, Rspo-2, Rspo-2+MaR1) were carried out in the same experiments (n = 3). For clarity, the results were separated into D and E. The same MaR1 response curve is presented in both panels for direct comparisons. The statistical analysis (2-way ANOVA with Tukey’s multiple comparisons test) was carried out with all 6 groups. (F) MaR1 (0.1–10 nM) was incubated with CHO-β-arrestin-LGR6 at 4°C, 25°C, 37°C, or 40°C. Results are mean ± SEM from 3 independent experiments. #P < 0.05, versus 4°C; **P < 0.01, versus 4°C and 25°C. (G) Intracellular cAMP. HEK cells transfected with human LGR6 or mock plasmids were incubated with 1- to 100-nM MaR1 for 15 minutes, and cAMP levels were determined. Results are mean ± SEM from 4 independent experiments. ***P < 0.001, versus HEK-mock cells; ###P < 0.001 versus vehicle control. (B–G) Statistical analysis was carried out using 2-way ANOVA with Tukey’s multiple comparisons test. (H) Maresin biosynthesis pathways.
Pathhunter Detection Kit, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pathhunter detection kit/product/DiscoverX corporation
Average 90 stars, based on 1 article reviews
pathhunter detection kit - by Bioz Stars, 2026-04
90/100 stars
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pathhunter® flash detection kit  (DiscoverX corporation)
90
DiscoverX corporation pathhunter® flash detection kit
(A) A panel of orphan GPCRs was screened in the presence of 10-nM MaR1 or vehicle (0.1% ethanol) using the β-arrestin <t>PathHunter</t> GPCR system. The % activity = 100% × (mean RLU of test sample – mean RLU of vehicle control)/(mean RLU of vehicle control). (B–E) Ligand (MaR1)-receptor interaction was monitored using the CHO-β-arrestin system overexpressing LGR6 or GPR148. Results are mean ± SEM from 3 independent experiments. (B) LGR6 or GPR148 cells with MaR1. #P < 0.05, ##P < 0.01. MaR1 versus vehicle with LGR6 cells. *P < 0.05, ***P < 0.001. LGR6 versus GPR148. (C) LGR6 cells with MaR1 or MaR1 ME. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. (D) LGR6 cells with MaR1, MCTR1, MCTR2, or MCTR3. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. (E) LGR6 cells with MaR1, Rspo-2, or Rspo-2+MaR1. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. For D and E, the 6 groups (MaR1, MCTR1, MCTR2, MCTR3, Rspo-2, Rspo-2+MaR1) were carried out in the same experiments (n = 3). For clarity, the results were separated into D and E. The same MaR1 response curve is presented in both panels for direct comparisons. The statistical analysis (2-way ANOVA with Tukey’s multiple comparisons test) was carried out with all 6 groups. (F) MaR1 (0.1–10 nM) was incubated with CHO-β-arrestin-LGR6 at 4°C, 25°C, 37°C, or 40°C. Results are mean ± SEM from 3 independent experiments. #P < 0.05, versus 4°C; **P < 0.01, versus 4°C and 25°C. (G) Intracellular cAMP. HEK cells transfected with human LGR6 or mock plasmids were incubated with 1- to 100-nM MaR1 for 15 minutes, and cAMP levels were determined. Results are mean ± SEM from 4 independent experiments. ***P < 0.001, versus HEK-mock cells; ###P < 0.001 versus vehicle control. (B–G) Statistical analysis was carried out using 2-way ANOVA with Tukey’s multiple comparisons test. (H) Maresin biosynthesis pathways.
Pathhunter® Flash Detection Kit, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pathhunter® flash detection kit/product/DiscoverX corporation
Average 90 stars, based on 1 article reviews
pathhunter® flash detection kit - by Bioz Stars, 2026-04
90/100 stars
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pathhunter prolabel detection kit  (DiscoverX corporation)
90
DiscoverX corporation pathhunter prolabel detection kit
(A) A panel of orphan GPCRs was screened in the presence of 10-nM MaR1 or vehicle (0.1% ethanol) using the β-arrestin <t>PathHunter</t> GPCR system. The % activity = 100% × (mean RLU of test sample – mean RLU of vehicle control)/(mean RLU of vehicle control). (B–E) Ligand (MaR1)-receptor interaction was monitored using the CHO-β-arrestin system overexpressing LGR6 or GPR148. Results are mean ± SEM from 3 independent experiments. (B) LGR6 or GPR148 cells with MaR1. #P < 0.05, ##P < 0.01. MaR1 versus vehicle with LGR6 cells. *P < 0.05, ***P < 0.001. LGR6 versus GPR148. (C) LGR6 cells with MaR1 or MaR1 ME. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. (D) LGR6 cells with MaR1, MCTR1, MCTR2, or MCTR3. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. (E) LGR6 cells with MaR1, Rspo-2, or Rspo-2+MaR1. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. For D and E, the 6 groups (MaR1, MCTR1, MCTR2, MCTR3, Rspo-2, Rspo-2+MaR1) were carried out in the same experiments (n = 3). For clarity, the results were separated into D and E. The same MaR1 response curve is presented in both panels for direct comparisons. The statistical analysis (2-way ANOVA with Tukey’s multiple comparisons test) was carried out with all 6 groups. (F) MaR1 (0.1–10 nM) was incubated with CHO-β-arrestin-LGR6 at 4°C, 25°C, 37°C, or 40°C. Results are mean ± SEM from 3 independent experiments. #P < 0.05, versus 4°C; **P < 0.01, versus 4°C and 25°C. (G) Intracellular cAMP. HEK cells transfected with human LGR6 or mock plasmids were incubated with 1- to 100-nM MaR1 for 15 minutes, and cAMP levels were determined. Results are mean ± SEM from 4 independent experiments. ***P < 0.001, versus HEK-mock cells; ###P < 0.001 versus vehicle control. (B–G) Statistical analysis was carried out using 2-way ANOVA with Tukey’s multiple comparisons test. (H) Maresin biosynthesis pathways.
Pathhunter Prolabel Detection Kit, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pathhunter prolabel detection kit/product/DiscoverX corporation
Average 90 stars, based on 1 article reviews
pathhunter prolabel detection kit - by Bioz Stars, 2026-04
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efc detection kit pathhunter prolabel/prolink detection kit  (Eurofins)
90
Eurofins efc detection kit pathhunter prolabel/prolink detection kit
(A) A panel of orphan GPCRs was screened in the presence of 10-nM MaR1 or vehicle (0.1% ethanol) using the β-arrestin <t>PathHunter</t> GPCR system. The % activity = 100% × (mean RLU of test sample – mean RLU of vehicle control)/(mean RLU of vehicle control). (B–E) Ligand (MaR1)-receptor interaction was monitored using the CHO-β-arrestin system overexpressing LGR6 or GPR148. Results are mean ± SEM from 3 independent experiments. (B) LGR6 or GPR148 cells with MaR1. #P < 0.05, ##P < 0.01. MaR1 versus vehicle with LGR6 cells. *P < 0.05, ***P < 0.001. LGR6 versus GPR148. (C) LGR6 cells with MaR1 or MaR1 ME. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. (D) LGR6 cells with MaR1, MCTR1, MCTR2, or MCTR3. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. (E) LGR6 cells with MaR1, Rspo-2, or Rspo-2+MaR1. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. For D and E, the 6 groups (MaR1, MCTR1, MCTR2, MCTR3, Rspo-2, Rspo-2+MaR1) were carried out in the same experiments (n = 3). For clarity, the results were separated into D and E. The same MaR1 response curve is presented in both panels for direct comparisons. The statistical analysis (2-way ANOVA with Tukey’s multiple comparisons test) was carried out with all 6 groups. (F) MaR1 (0.1–10 nM) was incubated with CHO-β-arrestin-LGR6 at 4°C, 25°C, 37°C, or 40°C. Results are mean ± SEM from 3 independent experiments. #P < 0.05, versus 4°C; **P < 0.01, versus 4°C and 25°C. (G) Intracellular cAMP. HEK cells transfected with human LGR6 or mock plasmids were incubated with 1- to 100-nM MaR1 for 15 minutes, and cAMP levels were determined. Results are mean ± SEM from 4 independent experiments. ***P < 0.001, versus HEK-mock cells; ###P < 0.001 versus vehicle control. (B–G) Statistical analysis was carried out using 2-way ANOVA with Tukey’s multiple comparisons test. (H) Maresin biosynthesis pathways.
Efc Detection Kit Pathhunter Prolabel/Prolink Detection Kit, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/efc detection kit pathhunter prolabel/prolink detection kit/product/Eurofins
Average 90 stars, based on 1 article reviews
efc detection kit pathhunter prolabel/prolink detection kit - by Bioz Stars, 2026-04
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pathhunter detection kit containing cell membrane permeabilizing reagent and beta-gal substrate  (DiscoverX corporation)
90
DiscoverX corporation pathhunter detection kit containing cell membrane permeabilizing reagent and beta-gal substrate
(A) A panel of orphan GPCRs was screened in the presence of 10-nM MaR1 or vehicle (0.1% ethanol) using the β-arrestin <t>PathHunter</t> GPCR system. The % activity = 100% × (mean RLU of test sample – mean RLU of vehicle control)/(mean RLU of vehicle control). (B–E) Ligand (MaR1)-receptor interaction was monitored using the CHO-β-arrestin system overexpressing LGR6 or GPR148. Results are mean ± SEM from 3 independent experiments. (B) LGR6 or GPR148 cells with MaR1. #P < 0.05, ##P < 0.01. MaR1 versus vehicle with LGR6 cells. *P < 0.05, ***P < 0.001. LGR6 versus GPR148. (C) LGR6 cells with MaR1 or MaR1 ME. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. (D) LGR6 cells with MaR1, MCTR1, MCTR2, or MCTR3. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. (E) LGR6 cells with MaR1, Rspo-2, or Rspo-2+MaR1. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. For D and E, the 6 groups (MaR1, MCTR1, MCTR2, MCTR3, Rspo-2, Rspo-2+MaR1) were carried out in the same experiments (n = 3). For clarity, the results were separated into D and E. The same MaR1 response curve is presented in both panels for direct comparisons. The statistical analysis (2-way ANOVA with Tukey’s multiple comparisons test) was carried out with all 6 groups. (F) MaR1 (0.1–10 nM) was incubated with CHO-β-arrestin-LGR6 at 4°C, 25°C, 37°C, or 40°C. Results are mean ± SEM from 3 independent experiments. #P < 0.05, versus 4°C; **P < 0.01, versus 4°C and 25°C. (G) Intracellular cAMP. HEK cells transfected with human LGR6 or mock plasmids were incubated with 1- to 100-nM MaR1 for 15 minutes, and cAMP levels were determined. Results are mean ± SEM from 4 independent experiments. ***P < 0.001, versus HEK-mock cells; ###P < 0.001 versus vehicle control. (B–G) Statistical analysis was carried out using 2-way ANOVA with Tukey’s multiple comparisons test. (H) Maresin biosynthesis pathways.
Pathhunter Detection Kit Containing Cell Membrane Permeabilizing Reagent And Beta Gal Substrate, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pathhunter detection kit containing cell membrane permeabilizing reagent and beta-gal substrate/product/DiscoverX corporation
Average 90 stars, based on 1 article reviews
pathhunter detection kit containing cell membrane permeabilizing reagent and beta-gal substrate - by Bioz Stars, 2026-04
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pathhunter® detection kit  (Eurofins)
90
Eurofins pathhunter® detection kit
(A) A panel of orphan GPCRs was screened in the presence of 10-nM MaR1 or vehicle (0.1% ethanol) using the β-arrestin <t>PathHunter</t> GPCR system. The % activity = 100% × (mean RLU of test sample – mean RLU of vehicle control)/(mean RLU of vehicle control). (B–E) Ligand (MaR1)-receptor interaction was monitored using the CHO-β-arrestin system overexpressing LGR6 or GPR148. Results are mean ± SEM from 3 independent experiments. (B) LGR6 or GPR148 cells with MaR1. #P < 0.05, ##P < 0.01. MaR1 versus vehicle with LGR6 cells. *P < 0.05, ***P < 0.001. LGR6 versus GPR148. (C) LGR6 cells with MaR1 or MaR1 ME. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. (D) LGR6 cells with MaR1, MCTR1, MCTR2, or MCTR3. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. (E) LGR6 cells with MaR1, Rspo-2, or Rspo-2+MaR1. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. For D and E, the 6 groups (MaR1, MCTR1, MCTR2, MCTR3, Rspo-2, Rspo-2+MaR1) were carried out in the same experiments (n = 3). For clarity, the results were separated into D and E. The same MaR1 response curve is presented in both panels for direct comparisons. The statistical analysis (2-way ANOVA with Tukey’s multiple comparisons test) was carried out with all 6 groups. (F) MaR1 (0.1–10 nM) was incubated with CHO-β-arrestin-LGR6 at 4°C, 25°C, 37°C, or 40°C. Results are mean ± SEM from 3 independent experiments. #P < 0.05, versus 4°C; **P < 0.01, versus 4°C and 25°C. (G) Intracellular cAMP. HEK cells transfected with human LGR6 or mock plasmids were incubated with 1- to 100-nM MaR1 for 15 minutes, and cAMP levels were determined. Results are mean ± SEM from 4 independent experiments. ***P < 0.001, versus HEK-mock cells; ###P < 0.001 versus vehicle control. (B–G) Statistical analysis was carried out using 2-way ANOVA with Tukey’s multiple comparisons test. (H) Maresin biosynthesis pathways.
Pathhunter® Detection Kit, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pathhunter® detection kit/product/Eurofins
Average 90 stars, based on 1 article reviews
pathhunter® detection kit - by Bioz Stars, 2026-04
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pathhunter® bioassay detection kit  (DiscoverX corporation)
90
DiscoverX corporation pathhunter® bioassay detection kit
(A) A panel of orphan GPCRs was screened in the presence of 10-nM MaR1 or vehicle (0.1% ethanol) using the β-arrestin <t>PathHunter</t> GPCR system. The % activity = 100% × (mean RLU of test sample – mean RLU of vehicle control)/(mean RLU of vehicle control). (B–E) Ligand (MaR1)-receptor interaction was monitored using the CHO-β-arrestin system overexpressing LGR6 or GPR148. Results are mean ± SEM from 3 independent experiments. (B) LGR6 or GPR148 cells with MaR1. #P < 0.05, ##P < 0.01. MaR1 versus vehicle with LGR6 cells. *P < 0.05, ***P < 0.001. LGR6 versus GPR148. (C) LGR6 cells with MaR1 or MaR1 ME. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. (D) LGR6 cells with MaR1, MCTR1, MCTR2, or MCTR3. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. (E) LGR6 cells with MaR1, Rspo-2, or Rspo-2+MaR1. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. For D and E, the 6 groups (MaR1, MCTR1, MCTR2, MCTR3, Rspo-2, Rspo-2+MaR1) were carried out in the same experiments (n = 3). For clarity, the results were separated into D and E. The same MaR1 response curve is presented in both panels for direct comparisons. The statistical analysis (2-way ANOVA with Tukey’s multiple comparisons test) was carried out with all 6 groups. (F) MaR1 (0.1–10 nM) was incubated with CHO-β-arrestin-LGR6 at 4°C, 25°C, 37°C, or 40°C. Results are mean ± SEM from 3 independent experiments. #P < 0.05, versus 4°C; **P < 0.01, versus 4°C and 25°C. (G) Intracellular cAMP. HEK cells transfected with human LGR6 or mock plasmids were incubated with 1- to 100-nM MaR1 for 15 minutes, and cAMP levels were determined. Results are mean ± SEM from 4 independent experiments. ***P < 0.001, versus HEK-mock cells; ###P < 0.001 versus vehicle control. (B–G) Statistical analysis was carried out using 2-way ANOVA with Tukey’s multiple comparisons test. (H) Maresin biosynthesis pathways.
Pathhunter® Bioassay Detection Kit, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pathhunter® bioassay detection kit/product/DiscoverX corporation
Average 90 stars, based on 1 article reviews
pathhunter® bioassay detection kit - by Bioz Stars, 2026-04
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chemiluminiescent pathhunter flash detection kit  (DiscoverX corporation)
90
DiscoverX corporation chemiluminiescent pathhunter flash detection kit
(A) A panel of orphan GPCRs was screened in the presence of 10-nM MaR1 or vehicle (0.1% ethanol) using the β-arrestin <t>PathHunter</t> GPCR system. The % activity = 100% × (mean RLU of test sample – mean RLU of vehicle control)/(mean RLU of vehicle control). (B–E) Ligand (MaR1)-receptor interaction was monitored using the CHO-β-arrestin system overexpressing LGR6 or GPR148. Results are mean ± SEM from 3 independent experiments. (B) LGR6 or GPR148 cells with MaR1. #P < 0.05, ##P < 0.01. MaR1 versus vehicle with LGR6 cells. *P < 0.05, ***P < 0.001. LGR6 versus GPR148. (C) LGR6 cells with MaR1 or MaR1 ME. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. (D) LGR6 cells with MaR1, MCTR1, MCTR2, or MCTR3. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. (E) LGR6 cells with MaR1, Rspo-2, or Rspo-2+MaR1. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. For D and E, the 6 groups (MaR1, MCTR1, MCTR2, MCTR3, Rspo-2, Rspo-2+MaR1) were carried out in the same experiments (n = 3). For clarity, the results were separated into D and E. The same MaR1 response curve is presented in both panels for direct comparisons. The statistical analysis (2-way ANOVA with Tukey’s multiple comparisons test) was carried out with all 6 groups. (F) MaR1 (0.1–10 nM) was incubated with CHO-β-arrestin-LGR6 at 4°C, 25°C, 37°C, or 40°C. Results are mean ± SEM from 3 independent experiments. #P < 0.05, versus 4°C; **P < 0.01, versus 4°C and 25°C. (G) Intracellular cAMP. HEK cells transfected with human LGR6 or mock plasmids were incubated with 1- to 100-nM MaR1 for 15 minutes, and cAMP levels were determined. Results are mean ± SEM from 4 independent experiments. ***P < 0.001, versus HEK-mock cells; ###P < 0.001 versus vehicle control. (B–G) Statistical analysis was carried out using 2-way ANOVA with Tukey’s multiple comparisons test. (H) Maresin biosynthesis pathways.
Chemiluminiescent Pathhunter Flash Detection Kit, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chemiluminiescent pathhunter flash detection kit/product/DiscoverX corporation
Average 90 stars, based on 1 article reviews
chemiluminiescent pathhunter flash detection kit - by Bioz Stars, 2026-04
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pathhunter detection kit 93e0001  (DiscoverX corporation)
90
DiscoverX corporation pathhunter detection kit 93e0001
(A) A panel of orphan GPCRs was screened in the presence of 10-nM MaR1 or vehicle (0.1% ethanol) using the β-arrestin <t>PathHunter</t> GPCR system. The % activity = 100% × (mean RLU of test sample – mean RLU of vehicle control)/(mean RLU of vehicle control). (B–E) Ligand (MaR1)-receptor interaction was monitored using the CHO-β-arrestin system overexpressing LGR6 or GPR148. Results are mean ± SEM from 3 independent experiments. (B) LGR6 or GPR148 cells with MaR1. #P < 0.05, ##P < 0.01. MaR1 versus vehicle with LGR6 cells. *P < 0.05, ***P < 0.001. LGR6 versus GPR148. (C) LGR6 cells with MaR1 or MaR1 ME. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. (D) LGR6 cells with MaR1, MCTR1, MCTR2, or MCTR3. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. (E) LGR6 cells with MaR1, Rspo-2, or Rspo-2+MaR1. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. For D and E, the 6 groups (MaR1, MCTR1, MCTR2, MCTR3, Rspo-2, Rspo-2+MaR1) were carried out in the same experiments (n = 3). For clarity, the results were separated into D and E. The same MaR1 response curve is presented in both panels for direct comparisons. The statistical analysis (2-way ANOVA with Tukey’s multiple comparisons test) was carried out with all 6 groups. (F) MaR1 (0.1–10 nM) was incubated with CHO-β-arrestin-LGR6 at 4°C, 25°C, 37°C, or 40°C. Results are mean ± SEM from 3 independent experiments. #P < 0.05, versus 4°C; **P < 0.01, versus 4°C and 25°C. (G) Intracellular cAMP. HEK cells transfected with human LGR6 or mock plasmids were incubated with 1- to 100-nM MaR1 for 15 minutes, and cAMP levels were determined. Results are mean ± SEM from 4 independent experiments. ***P < 0.001, versus HEK-mock cells; ###P < 0.001 versus vehicle control. (B–G) Statistical analysis was carried out using 2-way ANOVA with Tukey’s multiple comparisons test. (H) Maresin biosynthesis pathways.
Pathhunter Detection Kit 93e0001, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pathhunter detection kit 93e0001/product/DiscoverX corporation
Average 90 stars, based on 1 article reviews
pathhunter detection kit 93e0001 - by Bioz Stars, 2026-04
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lysis buffer from the pathhunter detection kit  (DiscoverX corporation)
90
DiscoverX corporation lysis buffer from the pathhunter detection kit
(A) A panel of orphan GPCRs was screened in the presence of 10-nM MaR1 or vehicle (0.1% ethanol) using the β-arrestin <t>PathHunter</t> GPCR system. The % activity = 100% × (mean RLU of test sample – mean RLU of vehicle control)/(mean RLU of vehicle control). (B–E) Ligand (MaR1)-receptor interaction was monitored using the CHO-β-arrestin system overexpressing LGR6 or GPR148. Results are mean ± SEM from 3 independent experiments. (B) LGR6 or GPR148 cells with MaR1. #P < 0.05, ##P < 0.01. MaR1 versus vehicle with LGR6 cells. *P < 0.05, ***P < 0.001. LGR6 versus GPR148. (C) LGR6 cells with MaR1 or MaR1 ME. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. (D) LGR6 cells with MaR1, MCTR1, MCTR2, or MCTR3. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. (E) LGR6 cells with MaR1, Rspo-2, or Rspo-2+MaR1. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. For D and E, the 6 groups (MaR1, MCTR1, MCTR2, MCTR3, Rspo-2, Rspo-2+MaR1) were carried out in the same experiments (n = 3). For clarity, the results were separated into D and E. The same MaR1 response curve is presented in both panels for direct comparisons. The statistical analysis (2-way ANOVA with Tukey’s multiple comparisons test) was carried out with all 6 groups. (F) MaR1 (0.1–10 nM) was incubated with CHO-β-arrestin-LGR6 at 4°C, 25°C, 37°C, or 40°C. Results are mean ± SEM from 3 independent experiments. #P < 0.05, versus 4°C; **P < 0.01, versus 4°C and 25°C. (G) Intracellular cAMP. HEK cells transfected with human LGR6 or mock plasmids were incubated with 1- to 100-nM MaR1 for 15 minutes, and cAMP levels were determined. Results are mean ± SEM from 4 independent experiments. ***P < 0.001, versus HEK-mock cells; ###P < 0.001 versus vehicle control. (B–G) Statistical analysis was carried out using 2-way ANOVA with Tukey’s multiple comparisons test. (H) Maresin biosynthesis pathways.
Lysis Buffer From The Pathhunter Detection Kit, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lysis buffer from the pathhunter detection kit - by Bioz Stars, 2026-04
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12 μl/well detection reagent (pathhunter detection kit  (DiscoverX corporation)
90
DiscoverX corporation 12 μl/well detection reagent (pathhunter detection kit
(A) A panel of orphan GPCRs was screened in the presence of 10-nM MaR1 or vehicle (0.1% ethanol) using the β-arrestin <t>PathHunter</t> GPCR system. The % activity = 100% × (mean RLU of test sample – mean RLU of vehicle control)/(mean RLU of vehicle control). (B–E) Ligand (MaR1)-receptor interaction was monitored using the CHO-β-arrestin system overexpressing LGR6 or GPR148. Results are mean ± SEM from 3 independent experiments. (B) LGR6 or GPR148 cells with MaR1. #P < 0.05, ##P < 0.01. MaR1 versus vehicle with LGR6 cells. *P < 0.05, ***P < 0.001. LGR6 versus GPR148. (C) LGR6 cells with MaR1 or MaR1 ME. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. (D) LGR6 cells with MaR1, MCTR1, MCTR2, or MCTR3. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. (E) LGR6 cells with MaR1, Rspo-2, or Rspo-2+MaR1. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. For D and E, the 6 groups (MaR1, MCTR1, MCTR2, MCTR3, Rspo-2, Rspo-2+MaR1) were carried out in the same experiments (n = 3). For clarity, the results were separated into D and E. The same MaR1 response curve is presented in both panels for direct comparisons. The statistical analysis (2-way ANOVA with Tukey’s multiple comparisons test) was carried out with all 6 groups. (F) MaR1 (0.1–10 nM) was incubated with CHO-β-arrestin-LGR6 at 4°C, 25°C, 37°C, or 40°C. Results are mean ± SEM from 3 independent experiments. #P < 0.05, versus 4°C; **P < 0.01, versus 4°C and 25°C. (G) Intracellular cAMP. HEK cells transfected with human LGR6 or mock plasmids were incubated with 1- to 100-nM MaR1 for 15 minutes, and cAMP levels were determined. Results are mean ± SEM from 4 independent experiments. ***P < 0.001, versus HEK-mock cells; ###P < 0.001 versus vehicle control. (B–G) Statistical analysis was carried out using 2-way ANOVA with Tukey’s multiple comparisons test. (H) Maresin biosynthesis pathways.
12 μl/Well Detection Reagent (Pathhunter Detection Kit, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/12 μl/well detection reagent (pathhunter detection kit/product/DiscoverX corporation
Average 90 stars, based on 1 article reviews
12 μl/well detection reagent (pathhunter detection kit - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


(A) A panel of orphan GPCRs was screened in the presence of 10-nM MaR1 or vehicle (0.1% ethanol) using the β-arrestin PathHunter GPCR system. The % activity = 100% × (mean RLU of test sample – mean RLU of vehicle control)/(mean RLU of vehicle control). (B–E) Ligand (MaR1)-receptor interaction was monitored using the CHO-β-arrestin system overexpressing LGR6 or GPR148. Results are mean ± SEM from 3 independent experiments. (B) LGR6 or GPR148 cells with MaR1. #P < 0.05, ##P < 0.01. MaR1 versus vehicle with LGR6 cells. *P < 0.05, ***P < 0.001. LGR6 versus GPR148. (C) LGR6 cells with MaR1 or MaR1 ME. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. (D) LGR6 cells with MaR1, MCTR1, MCTR2, or MCTR3. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. (E) LGR6 cells with MaR1, Rspo-2, or Rspo-2+MaR1. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. For D and E, the 6 groups (MaR1, MCTR1, MCTR2, MCTR3, Rspo-2, Rspo-2+MaR1) were carried out in the same experiments (n = 3). For clarity, the results were separated into D and E. The same MaR1 response curve is presented in both panels for direct comparisons. The statistical analysis (2-way ANOVA with Tukey’s multiple comparisons test) was carried out with all 6 groups. (F) MaR1 (0.1–10 nM) was incubated with CHO-β-arrestin-LGR6 at 4°C, 25°C, 37°C, or 40°C. Results are mean ± SEM from 3 independent experiments. #P < 0.05, versus 4°C; **P < 0.01, versus 4°C and 25°C. (G) Intracellular cAMP. HEK cells transfected with human LGR6 or mock plasmids were incubated with 1- to 100-nM MaR1 for 15 minutes, and cAMP levels were determined. Results are mean ± SEM from 4 independent experiments. ***P < 0.001, versus HEK-mock cells; ###P < 0.001 versus vehicle control. (B–G) Statistical analysis was carried out using 2-way ANOVA with Tukey’s multiple comparisons test. (H) Maresin biosynthesis pathways.

Journal: The Journal of Clinical Investigation

Article Title: Maresin 1 activates LGR6 receptor promoting phagocyte immunoresolvent functions

doi: 10.1172/JCI129448

Figure Lengend Snippet: (A) A panel of orphan GPCRs was screened in the presence of 10-nM MaR1 or vehicle (0.1% ethanol) using the β-arrestin PathHunter GPCR system. The % activity = 100% × (mean RLU of test sample – mean RLU of vehicle control)/(mean RLU of vehicle control). (B–E) Ligand (MaR1)-receptor interaction was monitored using the CHO-β-arrestin system overexpressing LGR6 or GPR148. Results are mean ± SEM from 3 independent experiments. (B) LGR6 or GPR148 cells with MaR1. #P < 0.05, ##P < 0.01. MaR1 versus vehicle with LGR6 cells. *P < 0.05, ***P < 0.001. LGR6 versus GPR148. (C) LGR6 cells with MaR1 or MaR1 ME. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. (D) LGR6 cells with MaR1, MCTR1, MCTR2, or MCTR3. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. (E) LGR6 cells with MaR1, Rspo-2, or Rspo-2+MaR1. *P < 0.05, **P < 0.01; ***P < 0.001 versus vehicle. #P < 0.05, ##P < 0.01; ###P < 0.001 versus MaR1. For D and E, the 6 groups (MaR1, MCTR1, MCTR2, MCTR3, Rspo-2, Rspo-2+MaR1) were carried out in the same experiments (n = 3). For clarity, the results were separated into D and E. The same MaR1 response curve is presented in both panels for direct comparisons. The statistical analysis (2-way ANOVA with Tukey’s multiple comparisons test) was carried out with all 6 groups. (F) MaR1 (0.1–10 nM) was incubated with CHO-β-arrestin-LGR6 at 4°C, 25°C, 37°C, or 40°C. Results are mean ± SEM from 3 independent experiments. #P < 0.05, versus 4°C; **P < 0.01, versus 4°C and 25°C. (G) Intracellular cAMP. HEK cells transfected with human LGR6 or mock plasmids were incubated with 1- to 100-nM MaR1 for 15 minutes, and cAMP levels were determined. Results are mean ± SEM from 4 independent experiments. ***P < 0.001, versus HEK-mock cells; ###P < 0.001 versus vehicle control. (B–G) Statistical analysis was carried out using 2-way ANOVA with Tukey’s multiple comparisons test. (H) Maresin biosynthesis pathways.

Article Snippet: Test compounds were incubated with cells for 1 hour at 37°C, and receptor activation was determined by measuring chemiluminescence using the PathHunter detection kit (DiscoverX). cAMP measurements.

Techniques: Activity Assay, Incubation, Transfection